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Dr. Yann Guerardel - Cross-reactivity of bacterial polysaccharides when diagnosing fungal infections - When Nocardia is betrayed by Candida

 

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Yann Guerardel

Laboratoire de Glygobiologie Structurale et Fonctionnelle, Lille

Cross-reactivity of bacterial polysaccharides when diagnosing fungal infections - When Nocardia is betrayed by Candida

Although they show very different prevalence rates, invasive fungal infections (IFI) and nocardiosis have been on the rise for several years. These infections are both characterized by very high mortality rates, due to their late identification, generally in immunodeficient patients. Indeed, establishing an early diagnosis is still difficult and the need for new tests is clearly a highly topical issue. The only two commercial serum-based diagnoses for Candida albicans IFIs are based on the recognition of parietal polysaccharides: beta-glucans for Fungitell® and mannans for PlateliaTM. During this presentation, I will outline our efforts to develop a new serum-based diagnostic test based on the detection of yeast metabolism-derived oligosaccharides by mass spectrometry. This robust method complements well existing tests with good clinical scores and advantageous implementation in a clinical environment. These studies have also allowed us to highlight the existence of a completely unexpected cross-reaction between the major polysaccharide of the nocardia cell-wall, an arabinogalactan with a structure that significantly differs from those of mycobacteria, and Fungitell®. We expect that this discovery may be exploited to improve the diagnosis of invasive nocardiosis.

Selected publications

  • Cornu et al. (2019) Evaluation of mass spectrometry detection of pan-fungal serum disaccharide (MS-DS) for the diagnosis of invasive fungal infections: results from a collaborative study involving six European clinical centres. J Clin Microbiol (2019) 57(5) pii: e01867-18
  • Mery et al. (2016) Application of mass spectrometry technology to early diagnosis of invasive fungal infections. J Clin Microbiol 54: 2786–2797
  • Sendid et al. (2015) Preliminary evidence for a serum disaccharide signature of invasive Candida albicans infection detected by MALDI Mass Spectrometry. Clin Microbiol Infect 21(1):88.e1-6

Contact: Anne Lemassu (anne.lemassu@ipbs.fr)

Note for visitors: Please come with a valid identity card

21 Jan

11:00 - 12:00

Seminar room - IPBS - Campus 205