Cell Biology
- Clonogenic cell survival assays
- Viability assays (SRB, CellTiterGlo, MTT, WST)
- Analysis of DNA end resection by flow cytometry
- Analysis of cell cycle progression and checkpoints
Molecular Biology
- Q-PCR
- site directed mutagenesis
- inducible shRNA expression
- (FP/FLAG/HA/Myc/His)-tagged proteins
- CRISPR/Cas9 technology for genome editing and DSB induction
Cell Imaging
- Biphoton laser microirradiation (DNA damage induction, live cell imaging for protein recruitment and protein mobility (FRAP))
- Reporter systems for DNA double-strand break repair (HR, End-Joining, SSA) in living cells
- High-resolution imaging of DNA repair complexes (Ku & DNA-PKcs foci)
- Super-resolution imaging of DNA repair complexes (3D-SIM & STORM)
- Imaging of clickable small molecules
Biochemistry
- Electrophoretic Mobility Shift Assay
- DNA-PK kinase assay
- DNA end synapsis assay
- In vitro end-joining reactions on linear plasmids
- Protein production (bacteria and insect cells) and purification
- Identification of drug targets by chemoproteomics
Telomere Analysis
- Telomeric FISH
- Shelterin Immunofluorescence
- IF-FISH
- Telomeric restriction fragments (TRF)
- STELA
- Telomerase assay