Tools and Skills

Cell Biology 

• Clonogenic cell survival assays
• Viability assays (SRB, CellTiterGlo, MTT, WST)
• Analysis of DNA end resection by flow cytometry
• Analysis of cell cycle progression and checkpoints

Molecular Biology 

• Q-PCR 
• site directed mutagenesis 
• inducible shRNA expression 
• (FP/FLAG/HA/Myc/His)-tagged proteins
• CRISPR/Cas9 technology for genome editing and DSB induction

Cell Imaging 

• Biphoton laser microirradiation (DNA damage induction, live cell imaging for protein   recruitment and protein mobility (FRAP)) 
• Reporter systems for DNA double-strand break repair (HR, End-Joining, SSA) in living cells
• High-resolution imaging of DNA repair complexes (Ku & DNA-PKcs foci)
• Super-resolution imaging of DNA repair complexes (3D-SIM & STORM)
• Imaging of clickable small molecules

Biochemistry 

• Electrophoretic Mobility Shift Assay
• DNA-PK kinase assay 
• DNA end synapsis assay
• In vitro end-joining reactions on linear plasmids
• Protein production (bacteria and insect cells) and purification
• Identification of drug targets by chemoproteomics

Telomere Analysis 

• Telomeric FISH 
• Shelterin Immunofluorescence 
• IF-FISH 
• Telomeric restriction fragments (TRF) 
• STELA 
• Telomerase assay